【摘要】目的  綜述國內外有關針刺戒毒的臨床療效和機理 的進展。方法  總結了耳針、體針、耳體結合舉治療方法在臨床運用中的療效，并簡要闡述了針刺戒毒的可能機制。結果  為針刺戒毒的臨床運用提供了可借鑒的資料。結論  針刺戒毒具有特效、安全、價廉、無明顯副作用的特點。

 

　　[Abstract] Objective  The author reviewed the advances of the clinical effect of acupuncture and its mechanism in stopping drug abuse in recent years. Method  The author summarized the curative effect of clinical application of various needling methods， such as auricular acupuncture, body acupuncture and the combination of auricular and body acupuncture. The possibility of stopping drug abuse by acupuncture was expounded. Result  To provide necessary data for the clinical application of acupuncture to stop drug abuse. Conclusion  In stopping drug abuse, acupuncture therapy is highly effective，safe，cheap and free of side effect.

 

　　摘要  目的：從大鼠脾臟克隆信號分子guanine-nueleotide-releastng factor (GRF) pleckstrin homofogy (PH)結構域基因并迸行谷胱肽轉移酶融合表達，為進一步尋找其新配基、研究其功 能打下基礎。方法：采用一步法從大鼠新鮮脾組織中提取反轉錄合成及方法擴大增目的基因片段，并克隆到pUC19中。引物末端標記后，以雙氧終止法測序。然后再克隆到表達載體PGEX-4T-1中作GST融合表達。結果:信號GRF的PH結構域基因完全正確，內部不含終止碼。并在表達載體pGEX-4T-1中獲得融合表達。結論：從組織細胞中克隆Ph結構域基因是可 行的方法，可在大腸桿菌中以GST融合蛋白的形式表達。

 

　　Abstract   AIM: To screen for novel ligands of the PH domains and to investigate the functions of the PH domains in signal transduction. METHODS: Rat spleens were employed to clone the gene encoding PH domain of GRF. Total RNA molecules were isolated from fresh sheens and mRNAs were reversely transcriped into cDNAs. After amplication by PCR, the fragments of DNA were cloned into vector pUC19. The method of dideoxy-termination was employed to sequence with labelled primmers, and then the gene was cloned into vector pGEX-4T-1 ?o express in fusion with GST. RESULTS：The sequence of gene was correct and the gene was successfully expressed in pGEX-4T-1. CONCLUSION: The methods of cloning PH domains of signaling molecules are feasible and useful in study of signal trans-duction. The fragments of the gene can correctly be expressed in E, Coli as fusion with GST.